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Amaç: Hedeflenmemiş antikanser ilaçlardan kaynaklı oksidatif stres çeşitli dokularda kardiyotoksisite, nörotoksisite ve hepatotoksisite gibi önemli yan etkilere neden olmaktadır. Paklitaksel, solid tümörlerin tedavisinde kullanılan taksan türevi kemoterapötik ilaçlardan biridir. Bu tür kemoterapötiklerin sinir hasarı oluşturduğu bilinmektedir. Çaydabulunan kateşinlerin en aktif bileşeni olan epigallokateşin gallat (EGCG) önemli bir antioksidan olup, DNA stabilitesinin sağlanılması ve sağlıklı yaşamda önemli bir role sahiptir. Çalışmamızda, paklitaksel ile oluşturulmuş oksidatif strese karşı epigallokateşin gallatın olası koruyucu etkileri incelenmiştir. Yöntem: Çalışma gruplarımızı oluşturan 32 sıçan her grupta 8 sıçan olacak şekilde 4 gruba ayrılmıştır. Deney protokolünün sonunda sıçanlar sakrifiye edilerek siyatik sinir dokusu izole edilmiştir. Sinir dokusu homojenize edildikten sonra süperoksitdismutaz (SOD), katalaz (KAT) enzim aktiviteleri ileglutatyon (GSH), malondialdehid (MDA) ve nitrik oksit (NO) seviyeleri incelenmiştir. Bulgular: Elde edilen verilere göre paklitaksel uygulamasının SOD, KAT aktivitesi ve GSH düzeylerini azalttığı MDA seviyesini artırdığı, bununla birlikte EGCG tedavisinin SOD, KAT aktivitesi ve GSH düzeylerini arttırdığı ve MDA seviyesini azalttığı bulunmuştur. Sonuç: Çalışmamızın sonucunda EGCG’in paklitakselin siyatik sinir dokusunda neden olduğu oksidatif stresi engelleyebileceği gösterilmiştir.
Objective: Oxidative stress caused by non-targeted anti-cancer drugs causes significant side effects such as cardiotoxicity, neurotoxicity and hepatoxicity in various tissues. Paklitaksel is one of the tax derivative chemotherapeutic drugs used in the treatment of solid tumors. This type of chemotherapy is known to cause nerve damage. Epigallocatechin gallate (EGCG) is an important antioxidant and plays an important role in ensuring DNA stability and healthy life. In our study, the possible protective effects of epigallocatechin gallate against oxidative stress created by paklitaksel have been studied. Method: 32 rats, which make up our working groups, are divided into 4 groups, in order to have 8 rats in each group. At the end of the test protocol, the siatic nerve tissue was isolated by sacrificing the mice. After the nerve tissue has been homogenized, the levels of hyperoxytic dysmutaze (SOD), catalase (KAT) enzyme activity (GSH), malondialdehyd (MDA) and nitric oxide (NO) have been studied. The findings: According to the data obtained, the application of paklitaksel has increased the MDA level in which the SOD, KAT activity and GSH levels are reduced, while EGCG treatment has increased the SOD, KAT activity and GSH levels and reduced the MDA level. The result: Our study has shown that EGCG can prevent oxidative stress caused by paklitaksin in the siatic nerve tissue.
Aim: Oxidative stress caused by untargeted anticancer drugs in tissues causes serious side effects such as cardiotoxicity, neurotoxicity, and hepatotoxicity. Paclitaxel is one of the taxane-derived chemotherapeutic drugs used in the treatment of solid tumors. Such chemotherapeutics are known to cause nerve damage. Epigallocatechin gallate (EGCG), which is the most active component of catechins in tea, is an important antioxidant and has an important role in ensuring DNA stability and healthy life. In this study, possible protective effects of epigallocatechin gallate against paclitaxel-induced oxidative stress were investigated. Method: 32 rats forming our study groups; divided into 4 groups with 8 rats in each group. As a result of the experimental protocol, the animals were sacrificed and sciatic nerve tissue was isolated. After nervous tissue homogenization, superoxide dismutase (SOD), catalase (KAT) enzyme activities and glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) levels were examined. Results: According to the data obtained, paclitaxel administration decreased SOD, CAT activity and GSH levels and increased MDA levels, however, EGCG treatment increased SOD, CAT activity and GSH levels and decreased MDA levels. Conclusion: As a result of our study, it was shown that EGCG can prevent the oxidative stress caused by paclitaxel in sciatic nerve tissue.
Alan : Sağlık Bilimleri
Dergi Türü : Ulusal
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