Özet:

In this work, a novel organoboron based monomer that was synthesis and analyzed on enzymatic biosensor systems. Utilizing the direct electropolymerization (one-step) method, an electrochemical enzymatic biosensor system was developed with novel organoboron polymer film-coated platinum screen printed electrodes/glassy carbon electrode. Electropolymerization of novel organoboron monomer was carried out through various electropolymerization conditions and the best conditions for biosensors systems were concluded. Organoboron polymer-based enzymatic and electrochemical analysis that will be developed in this thesis will be used for the determination of catechol that is one of the mostly analyzed phenolic compounds in the chemistry and agriculture industry. Catechol analysis was developed in which the tyrosinase enzyme was used as the sample determination system. With the developed biosensor system, the phenolic components were tested in the linear range between 1 µM to 200 µM with different electrodes. After the biosensor performance conditions optimization, real sample analysis were also performed for controlled catechol added green tea samples with 3 percent to 10 percent range of standard deviation results. Finally, it should be noted that the developed biosensor system can be designed and commercialized as a portable end product that allows real-time detection. Phenolic compounds, which are determined to determine the antioxidant and antimicrobial activities of natural foods, are partly made within the scope of quality control analysis, and the developed organoboron polymer-based biosensor system will allow faster, cheaper, precise, and real-time tests.

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