Özet:

Objective: Thiols are antioxidant organic compounds with sulfhydryl group. Dynamic thiol/disulphide homeostasis is considered a marker of oxidative stress. This study aimed to determine disulphide and thiol levels in patients with generalised stage III grade C periodontitis and to investigate the relationship between biochemical and clinical periodontal parameters, namely, bleeding on probing (BOP), clinical attachment level (CAL) and probing pocket depth (PPD). Materials and Methods: Forty-eight individuals participated in the study (control, n=23; periodontitis, n=25). BOP, CAL and PPD values of the groups were recorded before and after non-surgical periodontal treatment. Thiol disulphide homeostasis was assessed by an automatic and a new spectrophotometric method. Percentages of disulphide/total thiol, disulphide/native thiol and native thiol/total thiol were calculated. Results: The clinical periodontal parameters of the periodontitis group were higher (p<0.01) before treatment and lowered significantly after the treatment (p<0.05). Thiol levels were significantly lower (p<0.05) and disulphide levels were significantly higher in the periodontitis group (p<0.05). A significant negative correlation was found between native thiol and total thiol with all clinical periodontal parameters (p<0.05). A significant positive correlation was noted between % disulphide/thiol ratios with all clinical periodontal parameters before treatment (p<0.05). Conclusion: A significant correlation between the severity of periodontitis and serum total thiol and disulphide levels supported the hypothesis of oxidative stress in the etiopathogenesis of periodontitis.

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