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Bu çalışmanın amacı, Türkiye'deki buğday bitkileri ve rizosfer topraklarından patojenik ve patojenik olmayan Rhizoctonia tür ve anastomosis gruplarının (AG) türlerini belirlemek ve genetik çeşitliliklerini değerlendirmektir. Türkiye'nin Orta Anadolu Bölgesi'ndeki 5 ildeki buğday tarlalarının bitki ve rizosfer topraklarından Rhizoctonia türleri izole edilmiştir. İzolasyonlar sonucunda, toplam 88 adet çok çekirdekli (MN) ve iki çekirdekli (BN) Rhizoctonia izolatı elde edilmiştir. İzolatların teşhislerinde rDNA-ITS dizi analizi yöntemi kullanılmıştır ve MN Waitea circinata var. zeae, W. circinata var. oryzae, W. circinata var. circinata, MN Rhizoctonia solani AG 2-1, AG 2-2, AG 3, AG 4-HGII, AG 4-HGIII, AG 5, AG 8, AG 11 ve BN AG A, AG DI, AG E, AG G, AG H, AG I, AG I-benzeri ve AG K'ya ait oldukları belirlenmiştir. En çok izole edilen grup W. circinata var. circinata olmuştur. Patojenite çalışmalarında, en virulent grubun R. solani AG 4 olduğu saptanmıştır. BN izolatlar arasında, R. cerealis AG DI dışındaki diğer grupların patojen olmadığı tespit edilmiştir. İzolatların rDNA-ITS dizilerinden neighbor-joining filogenetik ağaçları oluşturulmuştur. Bu çalışmanın sonucunda, Türkiye’nin Orta Anadolu Bölgesi'ndeki önemli buğday üretim alanlarında MN ve BN Rhizoctonia AG izolatlarının bölgedeki dağılımı belirlenmiştir. Ayrıca, bu çalışma, bölgeden elde edilen Rhizoctonia AG izolatlarının genetik çeşitliliğinin moleküler bir yaklaşımla değerlendirildiği ilk kapsamlı çalışmadır.
The aim of this study is to identify and evaluate the genetic diversity of non-pathogenic and non-pathogenic Rhizoctonia species and anastomosis groups (AG) from grain plants and rizospheric soils in Turkey. The Rhizoctonia species have been isolated from the plants and rizospheric lands of the 5 provinces of the Middle Anadolu region of Turkey. As a result of the isolations, a total of 88 multi-core (MN) and two-core (BN) Rhizoctonia isolates were obtained. In the diagnosis of isolates, the rDNA-ITS series analysis method was used and there was MN Waitea circinata. We have Zee, W. Circinata. Oryzae has W. circinata. Circinata, MN Rhizoctonia solani AG 2-1, AG 2-2, AG 3, AG 4-HGII, AG 4-HGIII, AG 5, AG 8, AG 11 and BN AG A, AG DI, AG E, AG G, AG H, AG I, AG I and AG K. The most isolated group is W. circinata. It was circus. In pathogenic studies, the most virulent group was found to be R. solani AG 4. Among the BN isolates, other groups other than R. cerealis AG DI have been found not pathogenic. From the rDNA-ITS series of isolates are created neighbor-joining philogenetic trees. As a result of this study, the distribution in the region of MN and BN Rhizoctonia AG is determined in the significant grain production areas in the Central Anadolu Region of Turkey. Moreover, this study is the first comprehensive study in which the genetic diversity of Rhizoctonia AG isolates obtained from the region is evaluated by a molecular approach.
This study aims to determine the species and evaluate the genetic diversity of the pathogenic and nonpathogenic Rhizoctonia spp. and anastomosis groups (AG) from wheat plants and rhizosphere soils in Turkey. Rhizoctonia species were isolated from plants and rhizosphere soils in wheat fields in 5 provinces in the Central Anatolian Region of Türkiye. As a result of the isolations, a total of 88 multinucleate (MN) and binucleate (BN) Rhizoctonia isolates were obtained. Identifications of the isolates were determined by rDNA-ITS sequence analyses. The identified isolates belonged to MN Waitea circinata var. zeae, W. circinata var. oryzae, W. circinata var. circinata, MN Rhizoctonia solani AG 2-1, AG 2-2, AG 3, AG 4-HGII, AG 4-HGIII, AG 5, AG 8, AG 11 and BN AG A, AG DI, AG E, AG G, AG H, AG I, AG I-like and AG K. The most isolated group was W. circinata var. circinata. In the pathogenicity studies, the most virulent group was determined as R. solani AG 4. Among the binucleate isolates, groups other than R. cerealis AG DI were not found to be pathogenic. Neighbor-joining phylogenetic trees of isolates were constructed from rDNA-ITS sequences. As a result of this study, the regional distribution of MN and BN Rhizoctonia AG isolates in important wheat production areas in the Central Anatolia Region, Türkiye was determined. In addition, this study is the first comprehensive study in which the genetic diversity of Rhizoctonia AGs isolates obtained from wheat and rhizosphere soils in the region was evaluated with a molecular approach.
Field : Ziraat, Orman ve Su Ürünleri
Journal Type : Ulusal
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