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Bu çalışmada Crocus cancellatus subsp. mazziaricus (Herbert) Mathew geofit bitki ekstraktı iki farklı konsantrasyonda (0,5 ve 2 mg/balık) intraperitonal enjeksiyon yöntemiyle Avrupa deniz levreğine (Dicentrarchus labrax, L. 1758) uygulanmış ve bağışıklık sistemine etkisi araştırılmıştır. Enjeksiyon sonrası 7., 14., 21., 28. ve 35. günlerde balıklardan kan örnekleri alınmıştır. Doğal bağışıklık ile ilgili parametrelerden olan toplam lökosit sayısı, hematokrit seviye, NBT(+) hücre sayısı, lizozim aktivitesi ve serum total protein miktarındaki değişimler tespit edilmiştir. Crocus cancellatus subsp. mazziaricus uygulanan deneme gruplarında kontrol grubuna kıyasla doğal bağışıklık sisteminin aktifleşerek, NBT(+) hücre sayısının 21. günde, toplam lökosit sayısının 14.günde, serum total protein miktarının 14. ve 21. günlerde ve serum lizozim aktivitesinin 21. günde en yüksek seviyeye ulaştığı tespit edilmiştir. Sonuç olarak, 2 mg/balık dozunda uygulanan C. cancellatus subsp. mazziaricus ekstraktının 0,5 mg/balık dozuna kıyasla daha etkin olduğu bulunmuştur.
This is Crocus cancellatus subsp. Mazziaricus (Herbert) Mathew geofit plant extract was applied to the European seafood (Dicentrarchus labrax, L. 1758) by intraperitonal injection method in two different concentrations (0,5 and 2 mg/fish) and the effect on the immune system was studied. Blood samples were taken from fish on the 7, 14, 21, 28 and 35 days after the injection. Changes in the total number of leukocytes, hematocrit levels, NBT(+) cell numbers, lizosim activity and the total amount of serum protein have been detected from natural immunity parameters. Crocus cancellatus subsp. The natural immune system is activated compared to the control group in the trial groups applied to mazziaricus, with the number of cells NBT(+) in the 21st day, the total number of leukocytes 14. On a daily basis, the total amount of serum protein was found to reach the highest levels in the 14th and 21st days and the serum lizosime activity in the 21st days. As a result, C. cancellatus subsp is administered in 2 mg/fish dose. Mazziaricus extract has been found to be more effective than 0.5 mg/fish dose.
In this study, Crocus cancellatus subsp. mazziaricus (Herbert) Mathew geophyte plant extract was applied to European sea bass (Dicentrarchus labrax, L. 1758) in two different concentrations (0.5 and 2 mg/fish) by intraperitoneal injection method and its effect on the immune system was investigated. After injection on the 7th, 14th, 21st, 28th and 35th days, blood samples were collected from fish. Some of the innate immune related parameters in blood and serum samples such as total leukocyte count, hematocrit level, NBT (+) cell count, lysozyme activity and serum total protein content were determined. C. cancellatus subsp. mazziaricus plant extract treated groups exhibited significant immunostimulatory effect, increased NBT (+) cell count at 21st day, total leucocyte count in 14th day, serum total protein level at 14th and 21st day and serum lysozyme activity at 21st day compared with the control group. As a result of plant extract application, the 2 mg/fish group was found to be more effective than the 0.5 mg/fish group.
Alan : Fen Bilimleri ve Matematik; Ziraat, Orman ve Su Ürünleri
Dergi Türü : Uluslararası
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