Özet:

In this study in Kyrgyzstan for the first time in 2017 in Bishkek, Tuta absoluta's genetic characteristics were identified according to the sequence of the mitochondrial cytochrome oxidase subunit I (mtCOI) gen region. In the study, the Domates confidence collected from Bişkek was made DNA isolation from individuals and then the mtCOI area was amplified with specific primes. The sequence series obtained was created by comparing the 22 tomato seals sequence data obtained from GenBank. The analysis has shown that the data of the example of Kyrgyzstan and GenBank showed high similarity. The sequence data has been recorded to GenBank to be used in future studies. Research on the T. absoluta samples that will be collected from different molecular markers and other regions of Kyrgyzstan will be able to keep light on the genetic diversity, origin and future studies of harm.

Anahtar Kelimeler:

Özet:

In this study determination of the genetic trait of Tuta absoluta which was first determine in Kyrgyzstan in 2017, according to the sequence of mitochondrial cytochrome oxidase subunit I (mtCOI) gene region was aimed. In the study, DNA isolation was made from the tomato borer individuals collected from Bishkek and then the mtCOI region was amplified with specific primers and sequenced. The sequence data were compared with twenty-two tomato borer sequence data from GenBank and then phylogenetic tree were constructed. As a result of the analysis, it is seen that Kyrgyzstan sample showed high similarity with those GenBank data. Sequence data of Bishkek population were submitted to Genbank for futher studies. Research on the genetic diversity of T. absoluta samples collected from other regions Kyrgyzstan is thought to be beneficial for integrated pest management. Researches on T. absoluta samples collected from different regions of Kyrgyzstan with different molecular markers will be able to shed light on the genetic diversity, origin and future studies of the pest.