Özet:

This study aims to investigate the anti-inflammatory and antioxidant effects of chlorogenic acid, which is a powerful antioxidant in kidney ischemic-reperfusion damage experimentally created in rats. Tools and Methods: Thirty-six adult, Wistar albino sex female rats, randomly divided into four groups. The rats in the Sham group (Group I) were opened from the back area to the right nefrectomy. In the Iskemi-reperfusion group (Group II), the back area of the rats was opened and the left renal artery clamped after the right nefrectomy was performed. In Group III, 5 mg/kg of chlorogenic acid was administered intraperitoneally in the form of two doses, 10 minutes before and 10 minutes before reperfusion. A 24-hour reperfusion was applied to the left kidney after 1 hour ischemic. In Group IV, 10 mg/kg of chlorogenic acid was administered, unlike Group III. The total antioxidant levels (TAS), total oxidant levels (TOS), superoxid dismutaz (SOD), malondialdehitis (MDA) and myeloperoxidase (MPO) levels in the kidney tissue were measured by spectrophotometric methods. The oxidative stress index (OSI) is calculated. Results: In Group II, TOS, MDA and MPO levels increased compared to Group I, while TAS and SOD levels decreased (p<0.001 for each parameter). A decrease in the levels of kidney TOS (p<0.001), MDA (p<0.001) and MPO (p<0.001) in the groups applied chlorogenic acid, and an increase in the TAS (p<0.05) and SOD (p<0.001) values. The result is that oxidative stress plays an important role in kidney I/R damage and the application of chlorogenic acid against this damage has shown to significantly reduce the oxidative damage.

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