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Abstract Staphylococcus spp. isolated from samples of Minas cheese traditionally manufactured following artisan procedures were identified using molecular techniques and further analyzed using PCR and specific primers for the detection of classic enterotoxins (SEA, SEB, SEC, SED, and SEE) and toxic shock syndrome toxin-1 (TSST-1). Specific sea, sec, sed, and see genes were identified using multiplex PCR, whereas seb and tst genes were detected by uniplex PCR. In vitro antagonism with Lactobacillus spp. was evaluated to assess antimicrobial susceptibility. Classic enterotoxins and TSST-1 genes were not detected. The antimicrobials sulfonamide, penicillin, ceftzadime, and oxacillin showed higher resistance rates in the antibiogram (100%, 80%, 60%, and 40%, respectively), whereas other antimicrobials were effective in percentages above 70%. Lactobacillus spp. were able to inhibit Staphylococcus spp. in vitro. Thus, our results indicated that the isolated Staphylococcus spp. were sensitive to the most common antimicrobials tested and were inhibited by Lactobacillus spp. Author Biographies Dalila Lapinha Silva Oliveira Rosa, Universidade Federal de Minas Gerais Doutoranda em Ciência Animal Leonardo Borges Acúrcio, Universidade Federal de Minas Gerais Doutorando em Microbiologia Felipe Machado de Sant'Anna, Universidade Federal de Minas Gerais Mestrando em Ciência Animal Renata Dias de Castro, Universidade Federal de Minas Gerais Mestranda em Ciência Animal Bruno Oliver Rosa, Universidade Federal de Minas Gerais Doutorando em Zootecnia Sávio Henrique de Cicco Sandes, Universidade Federal de Minas Gerais Doutorando em Microbiologia Andreia Marçal da Silva, Universidade Federal de São João Del Rei Professora Adjunta da UFSJ Marcelo Resende de Souza, Universidade Federal de Minas Gerais Professor Adjunto da EV/UFMG Mônica Maria Oliveira Pinho Cerqueira, Universidade Federal de Minas Gerais Professora Associada da EV/UFMG Downloads PDF-PT (Português (Brasil)) Published 2015-02-03 How to Cite Rosa, D. L. S. O., Acúrcio, L. B., Sant’Anna, F. M. de, Castro, R. D. de, Rosa, B. O., Sandes, S. H. de C., Silva, A. M. da, Souza, M. R. de, & Cerqueira, M. M. O. P. (2015). Evaluation of toxicity genes, antimicrobial susceptibility, and in vitro antagonism of Staphylococcus spp. isolated from artisanal cheese. Health Surveillance under Debate: Society, Science & Technology, 3(1), 37–42. https://doi.org/10.3395/2317-269x.00226 More Citation Formats ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver Download Citation Endnote/Zotero/Mendeley (RIS) BibTeX Issue Vol. 3 No. 1 (2015): February Section Articles License Copyright (c) 2015 Health Surveillance under Debate: Society, Science & Technology (Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnología) – “Visa em Debate”

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