Abstract:

Aim: Staphylococcus aureus is a major cause of nosocomial and community-acquired infections. S. aureus attachment to medical implants and host tissue, and the establishment of a mature biofilm, play an important role in the persistence of chronic infections. The aim of our study was to determine the biofilm forming potential of methicillin-susceptible S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA) isolates and to observe the difference between them. Material and Methods: A total of 200 S. aureus strains (100 MRSA, 100 MSSA) obtained from various clinical samples sent to the Microbiology laboratory were included in our study. Identification of isolates was determined by Vitek MS (BioMerieux, France) device and antibiotic susceptibility was determined by Vitek2 Compact (BioMerieux, France) automated system.). Biofilm production was studied using the Microtitration plate method.The biofilm forming ability of MSSA and MRSA strains was statistically examined. Statistical significance level was taken as p<0.05. Results: We found 51% biofilm formation among all S.aureus strains included in the study. Biofilm formation was observed in 34% of MSSA strains and 68% of MRSA strains. There was a statistically significant difference in biofilm formation between MRSA and MSSA groups (p<0.05). Conclusion: We determined that the biofilm forming ability of MRSA strains was significantly higher than MSSA, in our study. MRSA and MSSA strains indicates that these strains have a high ability to persist in the hospital setting and increase the risk of disease development in hospitalized patients.