Abstract:

In this study, an enzymatic biosensor was developed with gold nanoparticles and its performance was tested for detection of phenolic compounds. Glassy carbon working electrode (GCE) was covered with different combinations of chitosan (Chit) as a support, gold nanoparticle (GNP) and tyrosinase enzyme (T) to form the enzymatic biosensor. The sensor components (Chit, GNP and T) were characterized by cyclic voltametry (CV) and electrochemical impedance spectroscopy (EIS) while its performance was tested by chronoamperometry method for catechol (as a model phenolic compound) selectivity. Increase in sensor signal was observed depending on high conductivity of gold nanoparticles. The developed sensor has wide linear range (0.046-50 µM), low detection limit (13.8 nM) and high sensitivity (1.144 A/M). The results indicate that this kind of biosensors is potential candidates for cheap, fast and simple detection of phenolic compounds. 

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