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Amaç: Bu çalışmada karbon tetraklorür (CCl4) ile oluşturulan deneysel karaciğer hasarında benfotiaminin karaciğer dokusu üzerine koruyucu etkilerinin incelenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmada, 30 adet Wistar albino cinsi erkek sıçanlar kullanıldı. Deney hayvanları 5 eşit gruba ayrıldı. Kontrol grubuna 14 günlük deney süresince herhangi bir uygulama yapılmadı. CCl4 grubuna ise 1. ve 8. günlerde 1ml/kg CCl4: zeytinyağı (1:2) intraperitoneal (i.p) verildi. CCl4 +benfotiamin grubuna 1. ve 8. günlerde iki defa 1 ml/kg CCl4: zeytinyağı (1:2) karışımı ve 70 mg/kg/gün oral benfotiamin verildi. Benfotiamin grubuna 70 mg/kg/gün oral benfotiamin verildi. Zeytinyağı grubuna 1. ve 8. günlerde 2 ml/kg zeytinyağı i.p. olarak verildi. Deney sonunda sıçanlar anestezi altında dekapite edildi. Karaciğer dokuları çıkarılıp incelemeler için parafin bloklar hazırlandı. Ayrıca malondialdehid (MDA) çalışması için dokular – 80 0 C de saklandı. Bulgular: Kontrol, benfotiamin ve zeytinyağı grupları arasında bakılan parametrelerde anlamlı bir farklılık yoktu. Kontrol grubu ile karşılaştırıldığında CCl4 grubunda, MDA, apoptozis ve bax immünreaktivitesinde anlamlı artış izlendi. CCl4 grubu ile karşılaştırıldığında CCl4 + benfotiamin grubunda ise MDA, apoptozis ve bax immünreaktivitesinde anlamlı azalma görüldü. Sonuç: CCl4’ün MDA, apoptozis ve bax immünreaktivitesini arttırdığı, tedavi olarak verilen benfotiaminin bu parametreleri azalttığı görüldü.
Purpose: The aim of this study was to compare the long-term results of the trocar and modified Seldinger techniques used in the percutaneous treatment of type CE1 and CE3a hepatic hydatid cysts. Materials and Methods: The data of the patients with CE1 and CE3a hydatid cysts, treated by the catheterization technique between January 2015 and June 2019 were reviewed retrospectively. Cyst type, cyst size and localization, treatment method applied, duration of catheterization and length of hospital stay were recorded. Follow-up period, cyst you at the last follow-up, and inactivation time were listed using the follow-up ultrasound reports. All of these variables were listed separately in the modified Seldinger and trocar techniques and compared statistically. Results: The data of 126 CE1 and CE3a patients were included in the study. The modified Seldinger technique was used in 36 patients, and the trocar technique was used in 90 patients. The average follow-up period was 1185.36 ± 426.02 days. The effectiveness of the treatment was at the rate of 97.2% in the modified Seldinger technique and 97.7% in the trocar technique. In total, recurrence was seen in 2.3% of the patients. The duration of catheterization and length of hospital stay were found to be significantly lower in the trocar technique compared to the modified Seldinger technique. The inactivation process was faster in the modified Seldinger technique. Both the modified Seldinger and trocar techniques are effective treatment modalities that can be used safely in the treatment of CE1 and CE3a hepatic hydatid cysts.
Purpose: In this study, we aimed to investigate the protective effects of benfotiamine on experimental liver injury caused by carbon tetrachloride (CCl4). Materials and Methods: In this study, 30 male Wistar albino rats were used. Rats were equally divided into 5 groups. No application was made to control group. The CCl4 group was injected i.p with1ml/kg CCl4:olive oil (1:2) mixture on the 1st and 8th days, and the CCl4+benfotiamine group was treated i.p with 1 ml/kg CCl4: olive oil (1:2) mixture twice on the 1st and 8th days and orally with 70 mg/kg/day benfotiamine. To the benfotiamine group, 70 mg/kg/day benfotiamine was given orally for 14 days. To the olive oil group, 2 ml/kg olive oil was given i.p. on 1st and 8th days. Finally, rats were decapitated. Liver tissues were removed and paraffin blocks were prepared. Tissues were stored at –80 oC for malonaldeyhde (MDA) assay. Results: There were no significant differences between the control, benfotiamine and olive oil groups. Compared with the control group, there was a significant increase in MDA, apoptosis and bax immunoreactivity in CCl4 group. Compared with the CCl4 group, there was a significant decrease in MDA, apoptosis and bax immunoreactivity in the CCl4+benfotiamine group. Conclusion: CCl4 increases MDA, apoptosis and bax immunoreactivity, and benfotiamine, given as treatment, reduces these parameters.
Alan : Sağlık Bilimleri
Dergi Türü : Uluslararası
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