Abstract:

Objective: The differential diagnosis of malignant mesothelioma (MM) and reactive mesothelial hyperplasia (RMH) is an important problem in effusion materials. Immunohistochemical antibodies are widely used to solve this problem. The purpose of this study is to determine the diagnostic value of GLUT-1, CD147 and ProExC in distinguishing MM from RMH. Material and methods: Immunohistochemical studies were performed on cell block sections from 26 cases of clinic and histopathologic confirmed MM and 26 RMH. The antibodies were evaluated semi-quantitatively based on the percentage and intensity of staining. The diagnostic performance of each antibody alone and combined was calculated. Results: Immunohistochemical GLUT-1 expression was seen in 25 of 26 (%96.1) MM and in 6 of 26 (%23) RMH. There was statistically significant difference between MM and RMH cases in terms of the percentage and intensity of staining with GLUT-1 (p <0.001). CD 147 was found positive in %100 of MM cases and RMH cases. There was not statistically significant difference between MM and RMH cases with CD147. The mean staining percentage of ProExC was found %14 in MM cases and %3.5 in RMH cases (p <0.001). The best cut-off value of ProExC in distinguishing MM from RMH was calculated as 7%. In conclusion GLUT-1 is a sensitive and specific immunohistochemical marker enabling differential diagnosis of MM from RMH. ProExC can be useful in differentiating MM from RMH. CD147 is not useful in the differential diagnosis of MM and RMH.