Özet:

Cancer is an important public health problem that is common throughout the world. Micro RNAs are small non-coding RNA transcripts that have important functions in the molecular mechanism of complex diseases such as cancer. In this study, it was aimed to reveal the effects of miR-96-5p on AIFM3 (apoptosis inducing factor mitochondria associated 3) gene and apoptosis in breast, lungs and osteosarcoma cancer cells. Materials and Methods: Osteosarcoma (U2-OS), breast (MCF7) and lung (A549) cancer cells were grown under normal culture conditions and subsequently subjected to miR-96-5p mimic transfection. Mimic transfected cells were harvested and miRNA and total RNA isolations were performed. Gene expression analyzes were performed by Real-Time PCR. Results: Expression of AIFM3 gene was shown to be significantly reduced in breast (p=0.015) and lung (p=0.024) cancer cells after miR-96-5p mimic transfections. In osteosarcoma cells, the expression level of the AIFM3 gene was shown to be decreased after miR-96-5p mimic transfections, but this change was not statistically significant. In addition, significant changes were found in the expression levels of apoptosis-related genes after mimic transfection in cancer cells. After miR-96-5p mimic transfection, the expression level of BCLxL (p = 0.043) and BCL2 (p = 0.0147) genes in MCF7 cells and BCL2 (p = 0.026) again in A549 lung cancer cells were found to be increased. Conclusion: Consequently, it was found that by increasing the expression level of miR-96-5p in cancer cells, the expression level of AIFM3 gene decreased and apoptosis-associated signaling was suppressed. Further comprehensive studies are needed to clearly understand the effects of miR-96-5p on apoptosis.

Anahtar Kelimeler: