Özet:

Objective: The preferred method for the purification of hematopoietic stem cells (HSCs), which can easily differentiate into blood cells, is the selection of CD34+ cells by flow cytometry or magnetic discrimination. Methods are needed that effectively determine human stem cells without entirely relying on phenotypic cell surface markers. One of these methods is cytosolic aldehyde dehydrogenase (ALDH), which plays a role in retinoid metabolism and resistance of HSCs to alkylating agents such as cyclophosphamide. The prospective isolation of human hematopoietic stem and progenitor cells with different functions, as well as surface markers, are also recommended by ALDH and flow cytometry. In the above findings, ALDH+ cells can be isolated from other cells and the most primitive hematopoietic stem cells can be isolated and used in clinical applications. Materials and methods: In our study, we separated the CD34 surface marker cells from cord blood using magnetic nanoparticles and then purified the cells with ALDH activity and non-CD38 surface antigen by flow cytometer. We visualized these cell groups with fluorescence microscopy. Results: We observed that the majority of CD34+ cells were ALDH+ in fluorescence microscopy and flow cytometry (FACS) analysis of CD34+ cells purified from cord blood as a result of ALDH-FITC markings. Conclusion: As a result of this study, it was found that the ALDH+ cells group is rich in CD34+ CD38- cells in hematopoietic stem cells isolated from cord blood, and is capable of colony formation in cell culture. It is suggested that in hematopoietic stem cell isolation from cord blood, only CD34+ cells are not sufficient and ALDH+ and CD38- negativity should also be analyzed. Keywords: Hematopoietic stem cells; cord blood; ALDH; CD34.

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