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Introduction: Pus, a whitish yellow liquid, is an accumulation of body’s defense mechanism produced during an inflammatory pyogenic infection due to bacteria. The overall incidence of wound sepsis in India is from 10% to 33%. This study was designed to evaluate the profile of aerobic pyogenic bacteria in our area along with their susceptibility to antimicrobial agents. Methodology: The current study was undertaken by the Department of Microbiology, Sri Krishna Medical College & Hospital, Muzaffarpur, Bihar, India. Pus samples were collected from in- and outpatients of various departments over a period of 2 years from March 2019 to February 2021. The specimens were either collected in sterile swabs or the pus was aspirated into sterile syringes and transported to the microbiology laboratory. These samples were processed on blood agar, chocolate agar, and MacConkey agar media and incubated at 37°C under aerobic conditions. The organisms were identified by biochemical reactions, Gram stain, and motility tests as applicable as per standard operative procedure. Prior approval was obtained from the Institutional Ethics Committee.The antimicrobial susceptibility tests were done by Kirby– Bauer’s disk diffusion method on Mueller–Hinton agar and interpreted as per Clinical Laboratory Standard Institution guidelines. Findings were tabulated and analyzed using SPSS ver. 21.0. Results: A total of 564 samples were tested of which 255 samples showed significant growth that constitute 45.2% of the total samples received during the study duration . Of these, 40.5% were gram positive cocci and 59.5% were gram negative bacteria. Of the gram-positive cocci, Staphylococcus aureus was the most prevalent organism, followed by Streptococcus pyogenes. S. aureus was highly resistant to penicillin, ampicillin, and erythromycin, and sensitive to vancomycin and linezolid. Of the 59.5% gram-negative bacilli isolated, Escherichia coli was the most common organism followed by Klebsiella, Pseudomonas, and others. Conclusion: On account of many antibiotics and their misuse, multidrug-resistant bacteria are emerging. Hence it becomes essential to know the prevalent profile and sensitivity pattern to guide the clinicians to start the empirical treatment.

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