Özet:

Epilepsy is a complex neurological condition associated with significant changes in psychological and emotional parameters affecting about 1% of the world’s population. Oxidative stress is a common way to explain neuronal damage in many neurodegenerative disorders A bioflavonoid has been to have neuroprotective, anti-inflammatory, analgesic, antibacterial, antihypercholesterolemic and anti-cancer properties. In our study, we aimed at exploring the effects of the combined application of Hesperidin and antiepileptic drugs of Valproic acid on the oxid-antioxidant defense in brain and liver tissue samples. For this purpose, 48 pieces of three-month 200-250 grams of male Wistar-Albino species were used. The experimental animals were divided into 6 groups, including 8 in each group: Group 1: Control, Group 2: Pentilentetrazole (PTZ) (60 mg/kg intraperitonal (i.p.)), Group 3: Hesperidin (HES) (100 mg/kg oral gathering), Group 4: PTZ + HES, Group 5: PTZ + Valproic acid (VPA) (400 mg/kg i.p.), Group 6: PTZ + VPA + HES. Chemicals were given to all groups outside the Control Group in 1-7-14-28 days and the activity of the superoxid dismutase (SOD) was determined with the levels of glutation (GSH) and malondialdehitis (MDA) in the tissue homogenates obtained at the end of the test. The desired results were subjected to statistical analysis with the Kruskal Wallis-H test in the SPSS program and the value of p≤0,05 was considered meaningful. Our study observed that the GSH levels and SOD activity in the PTZ given group decreased, and that the MDA levels also increased statistically significantly. The combined application of VPA and HES in the epilepsy model created with PTZ has been found to significantly increase the levels of brain and liver GSH and reduce the levels of MDA. In brain tissue samples, HES was observed to significantly increase the SOD activity compared to PTZ and PTZ+ VPA groups when given with VPA alone. In the liver tissue, HES has significantly increased SOD activity compared to the Control group, and in brain and liver samples, compared to the PTZ group, HES has significantly increased SOD activity compared to the Control group. Our findings show that when HES is used with antiepileptic therapy, it can increase antioxidant activity and reduce the possible side effects of VPA shown in some studies.

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