Özet:

Although its effects on health are not yet accurately known, electronic cigarettes have become popular worldwide in recent years. The lack of legal regulations causes an incoherence between the materials written on the product label and actually contained in the content. In this study, we aimed at analyzing the content of widespread e-fixes and their effects on two different cell lines. According to its availability and popularity, a dozen e-fluid samples were selected. Nicotine, propylene glycol (PG), glycerin (GLY) and flying compounds in e-flows were analyzed using high-performance fluid chromatography (HPLC) and gas chromatography (GC). The effect of E-fluids on cell vitality on the healthy liver cell line THLE-2 and pharyngeal carcinoma cell line Detroit 562 models was determined using 3-(4.5-dimetiltiyazolil-2)-2,5-difenyltetrazolyum bromide (MTT) vitality test. The quantities of nicotine were found to be compatible with the product labels. PG and GLY have shown differences not only between brands, but also for products within the same brand. The THLE-2 cell vitality was found in reverse proportion to the e-fluid concentration. However, reductions in cell vitality were not associated with the amount of nicotine. Interestingly, the effects of some samples on Detroit 562 cells were observed in three phases, including low-dose vitality reduction, cell survival in medium concentrations, and vitality loss in high-dose. The analytical composition of e-fluids shows great differences between the products that respond to different cellular effects. The fact that the vitality of cancer cells does not change depending on the dose, suggests that cell differences play a role in the results of these products.

Anahtar Kelimeler:

Özet:

Electronic cigarettes have become popular worldwide in recent years although their effects on human health are still not properly known. The lack of regulations brings a problem of inconsistency between ingredients and the product label. We aimed to analyse the contents of widely used e-liquids and their effects on two different cell lines. Eleven e-liquid samples were selected according to their availability and popularity. Nicotine, propylene glycol (PG), glycerine (GLY), and volatile compounds in e-liquids were analysed by High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay was used to determine the effects of e-liquids on transformed human normal liver epithelial cell line (THLE-2) and human pharyngeal carcinoma cell line (Detroit 562). Nicotine amounts were found to be consistent with product labels. and GLY were not only different between brands but also for products within the same brand. THLE-2 cell viability was inversely correlated with e-liquid concentration. However, decreases in cell viability were not correlated with nicotine amount. Interestingly, effects of several samples on Detroit 562 cells were triphasic; decrease in viability at lower doses, cell survival in mid-concentrations and loss of viability in highest doses. The analytical composition of e-liquids differs greatly among products which corresponds to different cellular effects. Viability of cancer cells does not change in a dose-dependent manner, which suggest that cellular differences may play role in the outcome of these products.