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Bu çalışmada, potansiyel bir kanser ilacı olan 2-tiyourasil (2-TU) ilacı ile balık sperminden elde edilen çift zincirli deoksiribonükleik asit (dsDNA) molekülü arasındaki etkileşim incelenmiş ve bu etkileşime dayalı olarak ilacın elektrokimyasal tayini gerçekleştirilmiştir. Bunun için, camsı karbon elektrot (GCE) yüzeyi, bromokrezol moru (BCP) monomerinin elektrokimyasal polimerizasyonu ile modifiye edilmiş ve bu elektrot (GCE/P(BCP)) yüzeyine, dsDNA elektrokimyasal olarak immobilize edilmiştir (GCE/P(BCP)/dsDNA). dsDNA ile 2-TU arasındaki etkileşim mekanizması diferansiyel puls voltametri yöntemiyle araştırılmıştır. Bu etkileşim sonrası guaninin yükseltgenme pik akımında azalma gözlenmiş ve bu azalmaya bağlı olarak 2-TU’in elektrokimyasal tayini indirekt yöntemle gerçekleştirilmiştir. 2-TU için doğrusal çalışma aralığı 0,1-50 mg L−1 ve gözlenebilme sınırı 0,033 mg L−1 olarak bulunmuştur. 2-TU−dsDNA etkileşim mekanizması UV-Görünür bölge moleküler absorpsiyon spektroskopi yöntemiyle de incelenmiştir. Hazırlanan DNA biyosensörüne bozucu etki yapabilecek türlerin etkisi araştırılmış ve ayrıca 2-TU ilacının idrar numunesinde tayini gerçekleştirilmiştir. Deneysel çalışmalardan elde edilen sonuçlara göre, 2-TU ve dsDNA arasındaki başlıca etkileşim modunun interkalasyon olduğu belirlenmiştir.
In this study, the interaction between the 2-tiyourassil (2-TU) drug, a potential cancer drug, and the double-chain molecule of deoxyribonucleic acid (dsDNA) obtained from fish sperm, was studied and on the basis of this interaction the electrochemical determination of the drug was carried out. For this purpose, the carbon electrode (GCE) surface was modified by the electrochemical polymerization of the bromocresol (BCP) monomerine, and the dsDNA was electrochemically immobilized (GCE/P(BCP)/dsDNA) to the surface of this electrode (GCE/P(BCP)) . The mechanism of interaction between dsDNA and 2-TU has been studied by the method of differential pulse voltameter. A decrease in the upgrading pic flow of this interaction has been observed and due to this decrease, the electrochemical determination of 2-TU has been carried out by indirect method. The linear work range for 2-TU was 0.1-50 mg L−1 and the observability limit was 0.033 mg L−1. 2-TU−dsDNA interaction mechanism is also studied by the method of UV-visible area molecular absorption spectroscopy. The effects of species that could have a disruptive effect on the prepared DNA bio-sensor have been studied and the prescription in the urine sample of the 2-TU drug has also been carried out. According to the results obtained from experimental studies, the main interaction mode between 2-TU and dsDNA was intercalation.
In this study, the interaction between 2-thiouracil (2-TU), a potential cancer drug, and double chain deoxyribonucleic acid (dsDNA) molecule obtained from fish sperm was investigated and electrochemical determination of the drug was performed. For this, the glassy carbon electrode (GCE) surface was modified by electrochemical polymerization of the bromocresol purple (BCP) monomer and dsDNA was electrochemically immobilized (GCE/P(BCP)/dsDNA) onto the surface of this electrode (GCE/P(BCP)). The interaction mechanism between dsDNA and 2-TU was investigated by differential pulse voltammetry method. After this interaction, a decrease in the oxidation peak current of guanine was observed and electrochemical determination of 2-TU was performed by indirect method due to this decrease. The linear operating range for 2-TU was 0.1-50 mg L− 1 and the detection limit was 0.033 mg L−1. The interaction mechanism of 2-TU − dsDNA was also investigated by UV-Visible molecular absorption spectroscopy. The effect of the species that may cause disruptive effect on the DNA biosensor was investigated and 2-TU drug was determined in the urine sample. According to the results obtained from experimental studies, the main mode of interaction between 2-TU and dsDNA is intercalation.
Alan : Fen Bilimleri ve Matematik; Mühendislik
Dergi Türü : Ulusal
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