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Dna Hasar Tespitinde Tek Hücre Jel Elektroforezi
2008
Journal:  
Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi
Author:  
Abstract:

Çeşitli iç ve dış nedenlerden dolayı DNA’da farklı düzeyde hasarlar meydana gelmektedir. DNA hasarına insan ve fare, at, domuz, balık gibi pek çok hayvan türünde rastlanmaktadır. DNA da oluşan bu hasarların başlıcaları; tek ve çift sarmal kırıkları, oksidatif DNA baz hasarı, DNADNA, DNA-protein ve DNA-ilaç çaprazlaşmaları ve DNA da mutasyonlar gibi bir takım yapısal bozulmalardır. Tek hücre jel elektroforezi yada “Comet Analiz” canlı populasyonlarında, hücre düzeyinde DNA hasar tespitininde kullanılan, hızlı, basit ve çok hassas flouresan mikroskobik yöntemdir

Keywords:

Dna Hasar Tespitinde Tek Hücre Yel Electrophoresis
2008
Journal:  
Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi
Author:  
Abstract:

Due to various internal and external causes, DNA damage occurs at different levels. DNA damage is found in many species of animals such as humans and mice, horses, pigs, fish. The main elements of these damage formed by the DNA are one-and-double slurry breaks, oxidative DNA base damage, DNA, DNA-protein and DNA-medicine crossings, and a set of structural deteriorations such as mutations. Single cell gel electrophoresis or "Comet Analysis" is a quick, simple and highly sensitive fluorescent microscopic method used in the detection of DNA damage in living populations at cell level.

Keywords:

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Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi

Field :   Fen Bilimleri ve Matematik; Mühendislik

Journal Type :   Ulusal

Metrics
Article : 1.428
Cite : 2.854
2023 Impact : 0.161
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Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi