Özet:

Objective: Difficulty in treating infections caused by positive microorganisms is one of the most important health problems today. The Gram negative GSBL positive bacteria, isolated from various bacteriological cultures, is intended to identify antibiotics effective for empirical and symptomatic treatment according to the sample type of bacteria. This study was conducted with a retrospective study of bacterial factors and antimicrobial sensitivity isolated from urine, blood, wounds, scraps, joint fluid, pleural fluid samples sent from various services and clinics to the routine microbiology laboratory between 1 August 2019 and 1 February 2020. Gram-negative GSBL positive bacteria and antibiotic sensitivity tests are performed by conventional method and MicroScan (Beckman Coultre, USA) automated system. Results: 1041 (60%) of 1735 gram negative bacteria isolated from 13,968 clinical samples sent to the laboratory was GSBL positive. 1041 for example; 901 (86,55) was in urine, 47 (4,51) was in Trakeal aspirat, 35 (3,36) was in wound. The most frequently isolated GSBL positive bacteria were 732(70.3%), Escherichia coli, 249(23.9%), Klebsiella pneumonia, and 22(2.11%) Proteus mirabilis. All of these bacteria were ampisillin, ampisillin-sulbactam and sefalosporin group resistant to antibiotics, while the most sensitive group of bacteria were carbapenem group antibiotics. The result: in Gram-negative bacteria, an increase in the proportions of antibiotically resistant isolated sulphur, significantly restricts the treatment options available. Specifically multi-antibiotic resistant, which often causes community and hospital-based infections, the determination of the antibiotic resistance conditions of Gram negative GSBL positive bacteria will help to increase the treatment success as it will reveal the current resistance rate. Each institution should contribute to the country’s data by determining its own antibiotic resistance.

Anahtar Kelimeler:

Özet:

Aim: The difficulties in the treatment of infections caused by Extended Spectrum Beta-Lactamase (ESBL) positive microorganisms are one of the most important health problems today. In this study, it was aimed to determine the antibiotics effective in empirical and symptomatic treatment according to the sample type of Gram negative ESBL positive bacteria isolated from various bacteriological cultures. Materials and Methods: This study was carried out by retrospective investigation of bacterial agents and antimicrobial susceptibility various samples sent to routine microbiology laboratory from various services and outpatient clinics between 1 August 2019 and 1 February 2020. Identification of Gram-negative ESBL-positive bacteria and antibiotic susceptibility tests were performed using the conventional method and MicroScan (Beckman Coultre, USA) automated system. Results: Of the 1735 Gram-negative bacteria isolated from 13,968 clinical samples sent to the laboratory, 1041 (60%) were ESBL positive. 1041 for example; 901 (86.55%) were urine, 47 (4.51%) were aspirate, 35 (3.36%) were wounds. Escherichia coli with 732 (70.3%) of ESBL positive bacteria, Klebsiella pneumonia with 249 (23.9%) and Proteus mirabilis with 22 (2.11%) were the most frequently isolated bacteria. While all of these bacteria were resistant to ampicillin-sulbactam, cephalosporin group antibiotics, the group to which the bacteria were most susceptible was carbapenem group antibiotics. Conclusion: Determining the antibiotic resistance status of Gram-negative ESBL-positive bacteria, which are resistant to multiple antibiotics and frequently cause infections, will reveal the current resistance rate and will help increase the success of treatment. Each institution should contribute to country data by detecting its own antibiotic resistance.