Abstract:

Aim: The aim of the study is to show the effectiveness of concentrated growth factor (CGF) and processed lipoaspirate (PLA) in wound healing in diabetic rats. Materyal and Method: A total of 30 rats were used in the study. İt was divided into 3 groups as CGF, PLA and control group. The rats were made diabetic using Sreptozotocin IP. A 5mm diameter wound was created on one of the hind legs of all rats by using a punch. CGF and PLA were applied to the lesions. Daily wound size and wound condition were recorded on days 3, 5 and 10. At the end of the study, blood samples were taken for TNF-α, TGF-β, IL-1, PDGF, FGF and VEGF measurements before the rats were sacrificed. Results: The mean wound diameters measured on the 3rd day in the study were 4.6 ± 0.06 mm in the control group, 4.1 ± 0.05 mm in the CGF group, and 4.4 ± 0.07 mm in the PLA group. The wound diameters measured on the 5th day were 3.1 ± 0.04 mm in the control group, 1.6 ± 0.05 mm in the CGF group and 2.7 ± 0.06 mm in the PLA group (P <0.01). The mean closure time of wounds was 5.3 ± 1.1 days in the CGF group, 7.1 ± 1.4 days in the PLA group, and 9.4 ± 0.5 days in the control group. All of the wounds were healed in all groups on the 10th day. This improvement rate in the CGF group was statistically significant compared to the other two groups (p<0.01). CGF and PLA increased the speed of wound healing in diabetic rats. Inflammatory marker levels (TNF-α, TGF-β, IL-1, PDGF, FGF, VEGF) obtained from blood samples were higher than normal in all rats and there was no significant difference between the groups (p>0.05). Conclusion: In this study, it was shown that CGF application was more effective than PLA application in wound healing in diabetic rats.

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