Abstract:

Ulserative colitis is a disease that occurs as a result of an excessive immune response to environmental factors in genetically sensitive people and the etiopathogenesis has not yet been fully illuminated. This study and the study of genetic expression differences between the region where inflammation is most intense and the biopsy materials taken from the normal colonial mucous are intended to identify biobelinders that may be associated with the occurrence and progress of the disease and thus contribute to the understanding of pathogenesis. Method:Gen expression differences in the inflammatory and normal mucous biopsy materials taken from 16 patients diagnosed with HIV have been studied using the Differential Display-PCR (DD-PCR), a partial transcriptom analysis method. The findings:Three of the candidate transcripts identified to be genetic expressions at different levels and the directory analysis was determined to be placed in the intronic areas of different genes in the genom. For one of these three transcripts, more detailed analysis was made. First, Rapid Amplification of cDNA Ends (RACE) experiments were conducted in order to identify the sequence of the cDNA ends of this transcript and the sequence of the 920 bc area of the 5th end was also identified. The analysis of genetic expression based on partial amounts was subsequently done using the designated primates of the area, but there was no significant difference between the inflammatory and normal colonic mucus in terms of genetic expression levels. Additionally, the RNA of 20 different tissues sold commercially and the RNA of 11 different cancer cell lines were used to make gen expression profiles, determined that the corresponding transcript expression occurred in many tissues and cancer cells. Result:This study resulted in a new transcript not previously identified by any other group. Further studies aim to determine the full size of this new transcript with the Northern Blot and perform functional analyses.