Abstract:

Purpose: Exposure to high doses of wholesale used in the industry can lead to a variety of symptoms as well as death. The aim of this study is to investigate changes in the post-mortem period in acetylcholinesterase, Na+/K+-ATPaz, phosphofructokinase and antioxidant enzyme (glutation peroxidase, catalase and hyperoxide dismutaz) activities in mice exposed to toluene, and to reveal possible clues about post-mortem. Method: In the study, the deadly doses of the injected rats were disqueated 0, 6, 12, 24 and 48 hours after death, collecting blood, brain and liver tissue. The enzyme activity of the prepared supernatins was analyzed. Results: In brain tissues, the enzyme activity of the test groups applied to toluen was reduced according to the control group for both AChE and PFK enzymes, while the Na+/K+-ATPaz activity was increased. Compared to the control group in antioxidant enzymes, the activity of three enzymes was increased. Plasma GSH-Px activity was observed to decrease 0-12 hours after death, and increased 24 and 48 hours after death. The activity of GSH-Px in the liver showed significant increases in the 0, 12 and 48 hours. CAT activity in the liver has increased significantly. There were no significant differences in the SOD levels. It is believed that the oxidative damage in the cells due to the Toluen effect has deteriorated the membrane structure, the three-dimensional structure of proteins and lipids and the cell repair mechanisms. Results: As a result, this study found that the oxidative damage associated with tolyne may lead to a change in certain enzyme amounts in the post-mortem process, which can also prove oxidative damage.

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