Abstract:

Hyperimmunized egg yolk (HIY) was produced in two layer hens by four successive immunization with live attenuated Newcastle disease vaccine (NDV) by seven days intervals for each vaccination process. Fifty broiler chicks were used for treatment with hyperimmunized yolk. They were divided into five groups. The first group was treated at 14th days of age by orally and intramuscular injection (five chicken for each route). The second group was boostered with (HIY) after 7 days of first dose at14 days of age. The third group as that of the first group but treated at 21st day of age. The fourth group was treated at 21st days and boostered after 7 days. The fifth group was ten chicks remain without any treatment used as control comparison of all groups. Immune response was measured using HI technique.The results showed that the group of 14th day of age with booster dose gave high antibody titer by intramuscular injection (second group).

Keywords: