This study aimed to survey the fish fauna of Cubatão River and its tributaries on the Atlantic Coast of São Paulo State, Brazil. For this study we captured the fishes in eight sampled sites: three in the Cubatão River itself (points 5, 6 and 7) and five in its tributaries, three on the left bank (1, 2 and 3) and two on the right bank (4 and 8) using an SAMUS 725M electric shock device, in a 50 meters area. Two samplings were done in rainy and dry season (2011). A total of six orders, nine families and 15 species, of which 53% were Siluriformes and 20% were Characiformes. The number of species collected in the middle Cubatão River basin represents 26.3% of the total richness recorded for coastal basins in the State of São Paulo (about 600 km of coastline).
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