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Bu çalışmada Liagora ceranoides (Rhodophyta), Halopteris scoparia (Ocrophyta), Padina pavonica (Ocrophyta) ve Sargassum vulgare (Ocrophyta) alglerinin etanol ve metanol ekstraktlarının hasta gökkuşağı alabalığı (Oncorhynhus mykiss)’ndan izole edilen Yersinia ruckeri suşları üzerine antibakteriyel aktiviteleri değerlendirilmiştir. Ayrıca, suşların çeşitli antibiyotiklere duyarlılıkları da çalışılmıştır. Balıklardan izole edilen suşlar fenotipik testler, API 20E hızlı tanı kiti ve PZR sonuçlarına göre Yersinia ruckeri olarak tanımlanmıştır. Çalışmada L. ceranoides, H. scoparia, P. pavonica ve S. vulgare makroalglerinin metanol ekstraktlarının Y. ruckeri’ye karşı antibakteriyel etki göstermediği bulunmuştur. P. pavonica’nın etanol ekstraktı Y. ruckeri’ye karşı düşük antibakteriyel aktivite göstermiştir. L. ceranoides, H. scoparia ve S. vulgare makroalglerinin etanol ekstraktlarının bakteriye karşı antibakteriyel aktivite göstermediği tespit edilmiştir. Antibiyogram test sonuçlarına göre suşların ampisilin, flumekuin, oksitetrasiklin ve trimetoprime duyarlı oldukları bulunmuştur. Suşlar eritromisine karşı orta derecede direnç göstermiştir. Sonuç olarak, alglerin antibakteriyel aktiviteye sahip olduklarının bildirilmesine karşın her alg türünün antibakteriyel aktivite gösteremeyeceği anlaşılmıştır.
This study assessed antibacterial activities on the Yersinia ruckeri juices isolated from the patient skeletal alabasis (Oncorhynhus mykiss) of the liagora ceranoides (Rhodophyta), Halopteris scoparia (Ocrophyta), Padina pavonica (Ocrophyta) and Sargassum vulgare (Ocrophyta) algae ethanol and methanol extract. Additionally, the sensitivity to various antibiotics has also been studied. Isolated spices from fish are defined as Yersinia ruckeri according to phenotypic tests, API 20E fast diagnosis kit and PZR results. The study found that L. ceranoides, H. scoparia, P. pavonica and S. vulgare macroalgs did not have an antibacterial effect on Y. ruckeri. P. Pavonica's ethanol extract Y. It has low antibacterial activity against ruckeri. L. ceranoides, H. scoparia and S. vulgare macroalgs have been found that ethanol extract does not show antibacterial activity against bacteria. According to the results of the antibiotic test, the juices were found to be sensitive to ampisillin, flumekuin, oxythracycline and trimetoprime. They showed a moderate resistance to erythromy. As a result, it was understood that, despite the reports that algae had antibacterial activity, it was not possible that every algae type showed antibacterial activity.
In this study, antibacterial activities of extracts of Liagora ceranoides (Rhodophyta), Halopteris scoparia (Ocrophyta), Padina pavonica (Ocrophyta) and Sargassum vulgare (Ocrophyta) on Yersinia ruckeri strains isolated from sick rainbow trout (Oncorhynhus mykiss) were evaluated. Also, susceptibilities of the strains against various antibiotics were studied. The strains isolated from the sick fish were identified as Yersinia ruckeri according to the results of the phenotypic tests, the API 20E rapid diagnostic kit and PCR. In the study, it was found that the methanol extracts of L. ceranoides, H. scoparia, P. pavonica and S. vulgare did not show antibacterial activity against Y. ruckeri. The ethanol extract of P. pavonica showed low antibacterial activity against Y. ruckeri. The ethanol extracts of L. ceranoides, H. scoparia and S. vulgare macroalgae did not show antibacterial activity against the bacterium. According to the results of the antibiogram test, it was found that the strains were sensitive against ampicillin, flumequine, oxytetracycline and trimethoprime. The strains showed intermediate resistance against erythromycine. In conclusion, although it was reported that algae had antibacterial activities, it was understood that every algae species could not exhibit antibacterial activity.
Alan : Sağlık Bilimleri
Dergi Türü : Ulusal
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