Özet:

Temperature and pH are important factors in plant tissue culture studies. The optimization of these factors is important for multiple production of plants. In this study, an optimization study for the efficient and rapid production of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne in different temperature (15-30°C) and pH (5.5-6.6) conditions was aimed. In temperature studies, the first shoot regeneration was observed at 25°C on the 12th day. The highest shoot regeneration frequencies (100%) were obtained at 20 and 25°C. The maximum number of shoots per explant (23.38) and the longest shoots (1.74 cm) were obtained at 25 °C. In different pH applications, the highest shoot regeneration rate (100%) was determined at pH 5.5 and 6. The maximum number of shoots per explant (20.44) and the longest shoot (1.71 cm) were recorded at pH 6. The explants usually gave longer shoots at higher pH values. After the shoots produced in the culture medium were rooted, they were successfully used in the aquarium environment. As a result, the optimum values for in vitro production of R. rotundifolia were found to be 25°C temperature and pH 6.0.

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