Özet:

The coronavirus (CoV), known for its existence for 70 years, was considered animal pathogens in the 1930s. In the 1960s, they were also identified as human respiratory pathogens. From that day to this day, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS) and finally severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) called epidemic diseases have emerged. This study aims to identify the phenotypic characteristics of host cells that play a role in the immune response to SARS-CoV-2. In this study, a total of 54 COVID-19 patients aged 18-71 were included. The immune phenotype analysis of T cells, B cells, Natural Killer (NK) cells, monocytes and monocytes-trombocytes aggregation was measured by the flow cytometry method using the FACSCalibur (Becton Dickinson, San Jose, CA, USA) device. Results: In 25.9% of patients, the number of lymphocytes was reduced, while in 74.1% the number of lymphocytes was normally. The CD3+ T-lenfocyte ratio was 73.96 ±7.96, the CD4+CD3+ T-Helper (Th) expression was 45,06 ±10,22, the CD8+CD3+ T-citotoxic (Tc) expression was 25,72 ±8,58%. The CD4/CD8 ratio was calculated at 1.89 ± 0.85. The CD19+ B lymphocytes rate was 9.82 ± 4.45%, and the CD56+ expression was 14.38 ± 6.28%. The HLA-DR ratio in lymphocytes was 16.41 ± 7.50%, while the HLA-DR ratio in monocytes was 92.31% (76.24-95,53). The CD61 expression in monocytes was 26 ± 13.95%. There was a negative correlation between CD61 and HLA-DR in monocytes (r= -0,347). The result: it is believed that we look at the immune phenotype characteristics of COVID-19 patients' T, B, NK and monocyte cells.

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