Özet:

This study includes the partial extraction of the alkaline phosfatase (ALP) enzyme extracted from the rats' liver, the study of its kinetic properties in the presence of inhibitors and inhibitors. With the help of experimental results, the graphics of Michaelis Menten and Lineweaver Burk were drawn. These graphs calculate the Km and Vmax values. Values: Inhibitors-free Km = 0,047 Vmax = 24,87; Urine inhibitors Km = 0,054 Vmax = 20,83; Creatine inhibitors Km = 0,037 Vmax = 20,83. According to these values, the enzyme's substratic relevance and maximum speed were determined. Experiments with the inhibitor were repeated and the type of inhibition was determined. It has been found how high urine and creatine levels in the blood affect the alkaline phosfatase enzyme. The study on ALP in the blood (Alkaline Phosphate) in which the product was used as an inhibitor found that the reaction was an uncompetitive (non-competitive) reaction, while the study in which creatine was used as an inhibitor found that the reaction occurred with an uncompetitive (non-competitive) reaction.

Anahtar Kelimeler: