Özet:

Aim: The purpose of this study was to investigate the effects of varying doses of Cefazolin on in vitro fibroblast culture. Material and Methods: In this study, the 3T3 mouse embryonic fibroblast cell line, which is widely used in in-vitro healing models, was used. Different doses of cefazolin, determined as 0 μg/ml, 50 μg/ml, 100 μg/ml, 250 μg/ml, 500 μg/ml and 750 μg/ml, were applied in-vitro on 3T3 mouse fibroblast cells. Experiments were planned as 24 and 48 hours. At the end of these periods, each group was evaluated with CCK-8 cell proliferation, migration and oxidative stress tests. Results: The IC50 values of the groups were found to be in the range of 500- 750 μg/ml at the end of the 24th and 48th hours, respectively. On the other hand, it was observed that cefazolin administered in the dose range of 50-500 μg/ml did not have a negative effect on cell proliferation. While it was determined that the migration rate decreased depending on dose and time, no significant difference was found in terms of oxidative stress in the experimental groups in which cefazolin was administered at different dose intervals. Conclusion: Cefazolin, administered pre- and postoperatively within therapeutic limits, did not cause any adverse effects on 3T3 mouse fibroblast cells in vitro.