Özet:

In almost every region of our country, roses are naturally grown or produced. There are many viral diseases that cause infection in the roses, and one of them is the rose yellow vein virus (RYVV). RYVV is a Rosadnavirus virus in the Caulimoviridae family. The virus has an ssDNA (+) genome consisting of 9,314 nucleotites. It is a new virus for our country and its characterization has not been made exactly. For this purpose, 71 rose samples with viral and viral symptoms have been collected in the 2018-2019 years in the province of Çanakkale. The samples collected were tested with a specific primary pair of protein genes with PCR (RYVV-F1 5'-CGAGA TTAAC GAAAT GGAC-3'and RYVV R1 5'-CGTAC TCTTT CTTGG CTT-3') The results of the tests found 71 of them infected. The selected one of the infectious isolates is concerned with the movement protein (movement protein; MP) and the coat protein (CP) genes are all amplified with PCR. Nucleotide series of MP and CP genes, which are amplified from the selected isolate using obtained PCR products, have been determined. Using the nucleotithic divisions of the obtained RYVV MP and CP genes, the amino acid divisions were removed. The multi-series comparisons made on the basis of nucleotid and amino acids found that there was a similarity of 96–98% in the level of nucleotid and 98–99% in the level of amino acids between Çanakkale RYVV isolate and the world’s isolates. Furthermore, philogenetic analyses have revealed the relationships of the world’s isolates with the Çanakkale RYVV isolate. With this study, for the first time in our country, molecular characterization of the RYVV Çanakkale isolate was carried out according to two genes.

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