Abstract:

The flavin-containing monooxygenase from Methylophaga sp. strain SK1 ( mFMO) is a valuable biocatalyst for the preparation of optically active sulfoxides, among other valuable compounds. In this study, we explored to benefits of using Natural Deep Eutectic Solvents (NADESs) when doing oxidation with this biocatalyst, fused to phosphite dehydrogenase for cofactor regeneration (PTDH- mFMO). It was found that optically active sulfoxides could be obtained with slightly higher conversions in 10% v/v NADES when working at substrate concentrations of 50–200 mM, whereas there was no loss in the enantioselectivity. With these results, it is demonstrated for the first time that flavin-containing monooxygenases can be employed as biocatalysts in presence of NADESs.

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