Abstract:

Restriction endonucleases are multifunctional enzymes that allow the target gen area to be extracted by cutting double-wire short DNA series from specific areas. These enzymes, also known as molecular bacteria, are studied in four general groups: type-I, type-II, type-III and homing endonucleases. Most of the enzymes used today are type-II endonucleases that can be cut from the full target area and are preferred especially in cloning studies. The RFLP technique is the process of dividing and visualizing DNA molecules into fragments of different sizes using restriction enzymes. Recombinate is a popular method used in obtaining DNA, creating restriction maps, determining population polymorphisms and diagnosing genetic diseases. In recent studies, it has often started to be preferred because it is a simple, fast and reliable technique in the diagnosis of protozoons and in the distinction of species.

Keywords: