Özet:

Purpose: Streptococcus pneumoniae is a pathogen that cause significant mortality and morbidity in humans. In particular, bacteremia, pneumonia, meningitis and middle ear inflammation. S.pneumoniae can cause many diseases in children and adults. Recently, S.pneumoniae is the most important cause of acute respiratory tract diseases in developing countries. Increasing antibiotic resistance in S.pneumoniae strains has recently gained importance. The aim of our study was to investigate the antimicrobial susceptibility and distribution of pneumococcal serotypes of S.pneumoniae strains isolated in our University Hospital.Methods: In this study we evaluated 80 S.pneumoniae strains isolated from adults and children between September 2012 and September 2013. The isolates were obtained from sputum n=56 , nasal secretions n=4 , throat swabs n=1 , blood n=9 , tracheal aspirat n=2 , conjunctival smear n=1 , bronchoalveolar lavage n=6 , and biopsy n=1 . Seventy-six isolates were from adults and four were from children. Antibiotic susceptibility testing to penicillin were performed on Mueller-Hinton agar supplemented with 5% sheep blood by the E-test method bioMerieux, France ; erythromycin, clindamycin, vancomycin, chloramphenicol, tetracycline, ofloxacine, levofloxacin, trimethoprim/sulfamethoxazole, rifampin, linezolid, and oxacillin susceptibilities performed by disc diffusion method and 7-10-13-valent Pneumotest-Latex Statens Serum Institude, Denmark kit was used for serotyping.Results: A total of 39/80 isolates 48.75% were intermediate, and 5/80 6.25% were resistant to penicillin according to Clinical & Laboratory Standards Institude CLSI guidelines for oral penicillin. Penicillin resistance was not found for parenteral penicillin. Resistance rates of erythromycin, clindamycin, vancomycin, chloramphenicol, tetracycline, ofloxacine, levofloxacin, trimethoprim/sulfamethoxazole, rifampin, and linezolid were as 31.25%, 21.25%, 0%, 5%, 28.75%, 15%, 2.5%, 45%, 0%, and 0% respectively. The most common S.pneumoniae serotypes were determined as serotypes 19, 6, and 23. Serotyping showed serotype 19 to be the leading serotype among the macrolide-resistant isolates, and serotype coverage of 7-10-13-valent pneumococcal vaccine was 68.75%.Conclusion: The increase in intermediate penicillin resistance in S.pneumoniae in our hospital should be monitored carefully and the distribution of pneumococcal serotypes is similar to countries where the Pneumococcal Conjugate Vaccine PCV has been introduced

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