Abstract:

In this study, different polarity solvent (hexane, ethyl acetate, ethanol and methanol) extracts of Alchemilla ellenbergiana (Lady's Mantle), which used in folk medicine plant in Gümüşhane region, were investigated in terms of total phenolic contents, antioxidant and antimicrobial activity.  Total phenolic contents were measured by spectrophotometric metod as gallic acid equivalent and detect in the highest in ethanol and methanol extracts. Antioxidant activity of extracts were determined by ABTS [2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] and DPPH (2,2-diphenyl-1-picrylhydrazyl) methods by spectrophotometric metod and calculated as trolox equivalent. Antimicrobial activity was tested against to pathogen microorganisms by disc diffusion method. As a result, it has been found that ethanol and methanol extracts of plant have high antioxidant and antimicrobial activity (p<0.05). Similarly, it has been found that antimicrobial activity of ethanol and methanol extracts higher than others.