Özet:

Accumulative reports documented that oxidative stress is implicated in many human and animal diseases. However, the reports concerning the effect of oxidative stress on pregnancy outcome are limited and scarce. The objective of this study was to determine the impact of oxidative stress on pregnancy outcome and to assess the antioxidant effect of vitamin C and E on oxidative stress parameters in blood and placental tissue samples in experimental pregnant animals model exposed to oxidative stress. Wister Albino rats were used in this work to investigate the effects of oxidative stress exposure (addition of H2O2 to the drinking water) on pregnancy outcome. Rats were divided into 5 groups, as follows: Group I (included 7 normal pregnant rats which served as control group). Group II (exposed to 1 % H2O2) included 7 pregnant rats, the rats were allowed to become pregnant and received (1% H2O2) in drinking water from day 7th till the day 19th of pregnancy. Group III (exposed to 3% H2O2) included 8 pregnant rats. Same as group 2, but the rats were exposed to a higher concentration of H2O2 (3%) in drinking water. Group IV (included 8 pregnant rats). Pregnant rats received vitamins C and E without induction of oxidative stress. Group V (included 8 pregnant rats).induction of oxidative stress by 1% H2O2 with vitamins supplementation in the pregnant rats. Serum total antioxidants capacity (TAC), serum and placental tissue oxidative stress biomarker; 8-iso prostaglandin F2α (8-Isoprostane) were measured using specific ELISA kits. Also placental tissues of pregnant rats were isolated and put directly in 10% formalin prepared for histopathological examination. Results revealed a significant decrease in the median values of the body weight and total serum antioxidants capacity (TAC) in groups II and III of rats compared with the control group. A significant higher median value of TAC obtained in the groups IV and V when compared with the control group. Significant higher levels of serum and tissue Isoprostane observed in both groups II and III compared with control group. Histopathological, oxidative stress induced macroscopically degenerative with microscopical appearance of vasculitis and hemorrhage within decidua. Data of the present study demonstrated that imbalance oxidative stress status in pregnant rats occurred due to exposure to oxidant, which played an important role in the pathogenesis of abnormal pregnancy outcome. In addition antioxidants supplementation (vitamins E and C) were valuable in reducing this stress.

Anahtar Kelimeler: