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Amaç: Behçet hastalığı (BH) genel olarak tekrarlayan oral aftlar, genital ülserasyonlar, deri lezyonları ve üveit ile karakterize olan kronik inflamatuar bir hastalıktır. BH’nın etiyopatogenezini aydınlatmayı hedefleyen pek çok genetik çalışma bulunmaktadır. Bu çalışmada IL-10’da bulunan rs1554286 tek nukleotid polimorfizmi (SNP) ve IL10 gen ekspresyonunun BH’ında incelenmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada DNA ve RNA izolasyonu için Behçet Hastaları (n=45) ve sağlıklı kontrollere (n=28) ait kan örnekleri toplanmış ve rs1554286 SNP genotiplemesi gerçek zamanlı PZR ile çalışılmıştır. Yeterli miktarda RNA elde edilen örneklerden (29 BH, 23 sağlıklı kontrol) cDNA elde edilerek IL-10 gen ekspresyonu yapılmıştır. Bulgular: IL-10 geni ekspresyon ve SNP genotiplemesi sonucunda BH ve sağlıklı kontroller arasında istatistiksel olarak anlamlı bir sonuç bulunmamıştır. Sonuç: Behçet hastaları ve sağlıklı kontroller arasında IL-10 geni ekspresyon ve SNP genotiplemesi karşılaştırması yapılan çalışmamızda iki grup arasında fark saptanmamıştır. Bu durum çalışmamızdaki örneklem sayısının azlığı nedeniyle ortaya çıkmış olabileceğinden daha yüksek örneklem sayılı çalışmalara ihtiyaç duyulmaktadır.
Purpose: BH is a chronic inflammatory disease that is characterized by generally recurring oral abnormality, genital ulcerations, skin lesions and uveitis. There are many genetic studies aimed at illuminating the etiopathogenesis of BH. This study aims to study rs1554286 single-nucleotid polymorphism (SNP) found in IL-10 and IL10 gen expression in the BH. Tools and Methods: This study collected blood samples for DNA and RNA isolation for Behcet patients (n=45) and healthy controls (n=28) and studied rs1554286 SNP genotyplemic with real-time PZR. A sufficient amount of RNA was obtained from the samples (29 BH, 23 healthy controls) with cDNA and IL-10 gen expression was made. Results: The result of IL-10 gen expression and SNP genotyplemic has not found a statistically meaningful result between BH and healthy controls. Results: There was no difference between the two groups in our study of IL-10 gene expression and SNP genotyplemic comparison between bacteria patients and healthy controls. This situation requires higher sample numbers than that may have occurred due to the lack of sample numbers in our study.
Objective: Behçet's disease (BD) is a chronic, recurrent and inflammatory disorder characterized by oral and genital aphthous ulcerations, uveitis, skin lesions and skin pathergy reaction. There are many genetic studies focused on determining BD etiology. In this study, we aimed to investigate IL-10 gene expression and rs1554286 single nucleotide polymorphism (SNP) which is located in IL-10 gene. Material and Method: In this study, the blood specimens of BD patients (n=45) and healthy controls (HC) (n=28) were collected for RNA and DNA extraction. We genotyped rs1554286 from the DNA samples (45 BD, 28 HC) using real time PCR. We identified the IL-10 gene expression levels from cDNA samples of patients with sufficient sample amounts (29 patients with BD and 23 HCs). Results: We compared the expression levels of IL-10 gene and also genotyping analysis, and no significant difference was observed between BD patients and HC. Conclusion: In our study, which compared IL-10 gene expression and SNP genotyping between Behçet patients and healthy controls, no difference was found between the two groups. Since this may have occurred due to the low number of samples in our study, studies with higher sample numbers are needed.
Field : Sağlık Bilimleri
Journal Type : Uluslararası
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