Özet:

This study was done to determine the philogenetic relationships in the Potentilla and to evaluate the importance of the trnL/trnF IGS region. Choosing the DNA barcode area to more effective, accurate and consistent results in the analysis is one of the most important points in the success of the study. For this purpose, on the basis of the trnL/trnF IGS sequence information of the chloroplast DNA obtained from NCBI, 71 Potentilla taxon was studied and MP dendrogram was drawn. The trnL/trnF IGS region has a suitable sequence length as a barcode area and it is also recommended to use this area with high variation and parsim info areas. When the trnL/trnF IGS area examined the possibilities of a base exchange from another base, the transitional base exchange was 57.13% higher than the transitional change. The MP dendrogram revealed that the taxons studied were placed in six groups: Fragarioides, Reptans, Anserina, Alba, Himalayan and Argentea. The study found that in Argentea, which is most represented by species, philogenetic relationships were quite complex in comparison with others. For the best results to be obtained, it is necessary to identify the areas that give the best results, and then to use together these areas that are determined with as many taxons as possible. As a result, it is recommended to use the trnL/trnF IGS sequence information that has a high variation area and a grouping capacity for the taxons studied.

Anahtar Kelimeler:

Özet:

This study was performed to determine the phylogenetic relationships in the genus Potentilla L. and to assess the importance of the trnL/trnF intergenic spacer (IGS) region. The selection of DNA barcoding region to provide for more effective, accurate and consistent results in the analysis is one of the most important points in success of the study. For this purpose, 71 Potentilla taxa were examined based on trnL/trnF IGS sequence data of chloroplast DNA acquired from the National Center for Biotechnology Information (NCBI) and a Maximum Parsimony (MP) dendrogram was drawn. The trnL/trnF IGS region has the convenient sequence length for barcoding region, and it is also proposed the use of this region which has highly variable and parsimony informative sites. When the probabilities of substitutions from one base to another base for trnL/trnF IGS region were examined, it was assigned that the rate of transitional substitutions with 57.13% is higher than the transversional substitutions. The MP dendrogram revealed that the studies taxa were placed in six groups as Fragarioides, Reptans, Anserina, Alba, Himalayan and Argentea. Phylogenetic relationships within the Argentea clade, represented by the highest species number in this study, were observed as quite confusing compared to other clades. In order to get the most accurate results, it is necessary to determine the regions giving the best results, and then to use these regions together with as many taxa as possible. In conclusion, the using of trnL/trnF IGS sequence information that have high variable sites and grouping ability for studies taxa is strongly recommended.