Abstract:

Investigation of drug–DNA interaction is important for understanding the drug action at molecular level and for designing specific DNA targeted drug. Furosemide is in a family of medicines called diuretics and is used for the treatment of edema caused by various medical problems, including diseases of the kidneys, heart and liver. The interaction of DNA with Furosemide has been analyzed by absorption, emission and Fourier transform infrared spectroscopic (FTIR). Results of FTIR indicate binding of Furosemide with guanine and cytosine nitrogen bases along with poor contact with the DNA sugar-phosphate backbone. UV and fluorescence spectroscopy studies of the interaction of Furosemide with DNA have shown that it can binds to DNA. For this reason, the interaction of the Furosemide drug molecule, which is used in many treatments, with DNA has been investigated and it has been determined that its binding to the DNA structure prevents DNA functions. These results suggest that the furosemide drug molecule may affect many DNA-based diseases.

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