Abstract:

The aim of this sutdy is to evaluate the effect of using the (IMBO) as probiotic in the reduction of diarrhea caused by E.coli in newborn calves. Using 150 newborn calves naturally infected with E.coli which is divided into three groups 50 animals in each group. First group, the animals of this group were inoculated Biomin IMBO as probioric orally given daily for 16 day with 6 gmanimal, while second group, the animals of this group were inoculated IMBO as probitic with same previous dose after natural infected with E.coli and suffering from diarrhea. While the third group (control group) which was not inoculated the IMBO as probiotic. The E.coli was isolated and it is virulence was evaluated by k99 pili test, the bacterial count for the feces sample of the calves was done as many hematological test which include (leukocyte count, differential WBCs count, packed cell volume, as well as TPP concentration and albumin and globulin for day (1,7,16) of the study. The probiotic was positively effective in the decrease of the severity of clinical sings and significantly increase calves weight which were treated by the probiotic, in the day (16) of the study and increase in the mortality rate compared with control group. The study shows probiotics use contributed in decrease of the number of E.coli excreted by feces of naturally infected calves with colibacillosis. Hematology test results showed significant increase in total leukocyte count, neutrophil, monocyte count and significant decrease in pact cell volume in the first and second group compared with control group. While serum test showed significant increase in total protein and globulin and significant decrease in albumin value. From the results of the study we notice the positive effect of probiotic IMBO on the health state after causing infection by colibacillosis and decrease the severity of clinical signs and increase the immunity respond.

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