Özet:

This study is aimed at experimental one-sided cryptoscopic examination of the electron microscopic and the relationship between testicular mast cells and fibrosis in mice. Tools and Methods: In our study, the rats were divided into two groups as control and cryptosides. The testicles were taken 15 days after the creation of the animal’s cryptorchidism model. Routine tracking procedures were applied to these tissues for light and electron microscopic examinations. H-E and TRI painted for light microscopic examinations. It was also evaluated the number of mast cells using anti-MCT antibodies for immune reactivity studies. The findings: In our study, the distinction of seminifer tubules in the instertical area in the cryptorchid testicles, the separation of germ cells from the basal membrane, perivascular fibrosis, swelling, conjunction, hemorrhage, increased fibrosis in the interstitial area and peritubular area, and a decrease in the diameter of seminifer tubules. In the electron microscope, severe degenerative changes in seminifer tubules and insterstysum, the basal lamina thickness increased significantly, in the sitoplasms of the Sertoli cells, the mytochondrions with intense SER vacualization and an abnormal crystal, the close connections between the Sertoli-Sertoli cells and the Sertoli-spermatoogenic cells and the cytoplasmic bridges of the spermogenic cells lost the integrity between them. A positive correlation was found between fibrosis in the peritubular area and the number of mast cells. The result: The experimental model of cryptorchidism in our study found that there was a positive correlation between the number of mast cells and fibrosis. Keywords: Electron Microscopic Structure; Fibrosis; Cryptorchidism; Mast Cell ABSTRACT Objective: In this study, it was aimed to investigate the relationship between electron microscopic examination and testicular mast cells and fibrosis in rats with experimental unilateral cryptorchidism. Material and Methods: In this study, rats were divided into two groups as control group and experimental group with cryptorchidism. The testes of the rats were extracted fifteen days after the Cryptorchidism model had been developed in rats. These tissues were processed according to standard methods for the light and electron microscopic analysis. TRI and H-E stains were used for light microscopic examinations. Also, the mast cells were evaluated by using anti-MCT anticors for immuneractivity studies. Results: In our study, the diverge of seminiferous tubules from each other in interstitial space, separation/disconnection of germ cells from basal membrane,perivascular fibrosis,edema, congestion,hemorrhage, the increament in the fibrosis in inertitial space and peritubules space, and the decreament in the diameters of seminiferous tubules were observed in tests with cryptorchidism. Electron microscopic analysis revealed levels degenerative changes in seminiferous tubules and interstisium, significant increase in the thickness of basal lamina, intense SER vacuolization and mitochodrions with anormal crista in the cytoplasm of sertoli cells, destruction in tight junctions between the sertoli cells each other and also sertoli cells and spermotogenic cells, decomposition of cytoplasmic bridges between the spermatogenic cells. The possitive correlation was detected between the number of the mast cells and fibrosis in peritubuller space. Conclusion: The positive correlation was detected between the number of the mast cells and fibrosis in the experimental cryptorchidism model. Keywords: Electron Microscopic Structure; Cryptorchidism; Mast Cell

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