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Termofilik bakteriler sıcak çevrelerde gelişebilen mikroorganizmalardır. Bu bakterilerin ürettikleri enzimler bitki ve hayvan kaynaklı enzimlere göre daha stabil olabilmekte ve daha ucuz olarak elde edilmektedirler.Termostabil enzimler ekstrem şartlarda esneklik ve tolerans göstermektedirler. Lipazlar biyoteknoloji ile ilişkili önemli gruplardır ve gıda, mandıra, deterjan ve ilaç endüstrisinde kullanılmaktadırlar. Diğer önemli bir enzim grubu olan amilazlar kağıt, şeker, tekstil, deterjan, fırında pişirme ve demleme endüstrisi gibi çeşitli alanlarda kullanılmaktadır. Proteazlar hidrolitik enzimlerdir ve deterjan üretimi, deri ürünleri, ilaç, et yumuşatıcı olarak, gıda ürünleri ve katı atık arıtma sanayii gibi çeşitli endüstriyel işlemlerde kullanılmaktadır. Bu çalışmada, Aydın ve Denizli’deki bazı termal alanlardan elde edilen 30 suşun 16S rDNA bölgeleri PCR ile çoğaltılmış ve moleküler tanıları yapılmıştır. Otuz izolat arasında suşların %50’si lipaz, %63.3’ü amilaz, %56.6’sı proteaz enzimlerini üretmektedirler. Lipaz-proteaz üreten üç, amilaz-proteaz üreten yedi izolat saptanırken, her üç enzimi de üreten 6 izolat olduğu belirlenmiştir.Sonuç olarak, çalışmamızda elde edilen suşlar ve ürettiklerienzimler endüstriyel biyoteknolojide kullanılma potansiyeline sahiptir.
Thermophilic bacteria are microorganisms that can develop in hot environments. The enzymes produced by these bacteria can be more stable and cheaper than the plant and animal enzymes.Termostabil enzymes show flexibility and tolerance in extreme conditions. Lipazes are important groups associated with biotechnology and are used in the food, mandra, detergents and pharmaceutical industries. Another important enzyme group, amylases are used in a variety of fields such as paper, sugar, textile, detergent, oven cooking and boiling industry. Proteases are hydrolytic enzymes and are used in various industrial processes such as the production of detergents, skin products, medicines, meat milder, food products and solid waste treatment industry. In this study, the 16S rDNA areas of 30 shells obtained from some thermal areas in Aydin and Denizli were reproduced with PCR and molecular diagnoses were made. Between thirty isolates, 50% of the juices produce lipase, 63.3% amylase, and 56.6% protease enzymes. Three which produce lipase-protease, and seven which produce amylase-protease, are identified as 6 which produce each of the three enzymes.As a result, the sulphes and produced enzymes obtained in our study have the potential to be used in industrial biotechnology.
Thermophilic microorganisms are able to grow at high temperatures. Enzymes produced by these kind of bacteria are more stable and cost effective. Thermostable enzymes have flexibility and tolerance in extreme conditions. Lipases dealing with biotechnology are important groups of enzymes. These enzymes are used in food, dairy, detergent and pharmaceutical industry. Amylases that are also important groups are used in various biotechnological fields such as paper, sugar, textile, detergent, baking and infusion industry. Proteases are hydrolytic enzymes and they are used in various industries such as producing detergent, leather products, pharmaceutical, meat tenderizer, food products and solid waste treatment industry. In this study, 16S rDNA regions of thirty bacterial strains isolated from thermal areas in Aydin and Denizli are amplified by PCR and isolates were identified by molecular methods. Among 30 isolates, percentage of strains that produce one enzyme is that; lipase %50, amylase %63.3, protease %56.6. Three of strains were found to be produce both Lipase and protease, while seven strains were amylase and protease producers. Six strains were found to capable of producing all three enzymes. As a result, isolates obtained in our study and their enzymes have an important potential in industrial biotechnology.
Alan : Fen Bilimleri ve Matematik; Mühendislik
Dergi Türü : Uluslararası
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