Abstract:

Purpose: Morphologically similar but immunologyK, molecular biologyK and biochemical characteristics geneticly different entamoeba entamoeba histolytica (E. histolytica) form amibic colitis and liver apses, while the other type, E. dispar, has non-invasive characteristics. Especially by laboratory workers with not sufficient experience, it is mixed directly in the microscope with E. histolytica/dispar; leukocytes, macrophages and other trophosites. However, as the factor is E.histolytica in the cases of amygdala disantery, the precise diagnosis is of great importance in the treatment planning. Our study assessed the use of the ELISA method for routine diagnostic purposes, based on the essence of search for the E. histolytica adezin antigen in the output. Method: 553 output samples sent to the laboratory for routine parasitological examination were studied microscopically by making native lugol method and trichrom coloring. The presence of adezin antigen in microscopically observed E histolytica/dispar cysts is studied using the specific monoclonal ELISA (E.HISTOLYTICA II Techlab, Blacksburg VA 24060, USA) method. In statistical analyses, Fisher ki-core test was used. The findings: Nativ lugol painting method identified E.histolytica/E.dispar cysts and/or trophosite structures in 29 (5.2%) output samples with Trikrom painting in 22 (3.9%) E.histolytica/E.dispar cysts and/or trophosite structures. In 15 of the samples (2.7%) with the ELISA method, the adezin antigen was positively identified. According to the ELISA method, the sensitivity of the trichrome paint method was 86.6%, the specificity was 35.7%, the positive predictive value was 59%, the negative predictive value was 71%. The statistical difference between the two methods has been significantly determined in terms of the diagnosis of invasive E. histolytica (p<0.05). The result: in the distinction between pathogen E.histolytica and non-patogen E.dispar, specific monoclonal ELISA adhesive antigen test to E.histolytica is necessary to reduce unnecessary therapeutic applications.