Özet:

Objective: The goals of this study are to evaluate the changes in tissue factor (TF) activities and sialic acid (SA) concentrations of D-galactose induced rat liver and renal tissues and compare with the naturally aged and the control groups.  Methods: In this study Sprague-Dawley male rats (n=28, 20 weeks old) were used. The experiment and the control groups were organized as follows: 1) young male control group (n= 8, 20 weeks old). 2) naturally aged male group (n= 10) 3) D-galactose induced ageing group (D-galactose injected (60 mg/kg/day) for 6 weeks, n= 8, 20-week-old). At the end of the treatment, the rats were sacrificed following a overnight fast, kidney and liver tissues were immediately collected for homogenization. TF activity and SA levels were measured by the methods of Quick and Warren respectively. Results: SA levels of kidney decreased whereas TFa (TF activity) increased significantly in the D-galactose-induced aging group when compared with the control and the naturally aged groups. SA levels of liver did not change significantly between the groups. TFa of the liver increased significantly compared to the control group. Conclusion: Increased TFa (TF activity) of kidney and liver and decreased SA levels of kidney in D-galactose induced aging model show that this model can be used in studies related with aging, thrombosis and hemostasis. Key words: D-galactose, aging, tissue factor, sialic acid

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