Abstract:

This study was carried out to detect Babesia spp. prevalence by Reverse Line Blotting (RLB) test and microscopic examination on sheep and goats around Yeşilhisar in Kayseri region. For this purpose, the blood samples were randomly collected into the tubes with EDTA from a total of 300 healthy small ruminants that consisted of 200 sheep and 100 goats in Yeşilhisar its and vicinity. Blood samples were investigated against Catch-all, Babesia ovis and Theileria ovis using RLB test. In addition, peripheral blood smears were prepared and examined under the microscope. In the microscopic examination, piroplasms were determined in 28 (14%) of 200 sheep, 5 (5%) of 100 goats and totally 33 (11%) of 300 small ruminants. It was also determined that 9 (27.3%) of them had Babesia spp. and 24 (72.7%) of them had Theileria spp. In 300 small ruminants, Catch-all positive was found in 99 (49.5%) of 200 sheep and 18 (18%) of 100 goats, totally 117 (39%) of 300 using RLB. It was determined T. ovis in 91 (92%), B. ovis in 3 (3%) and B. ovis+T. ovis in 5 (5%) of 99 Catch-all positive sheep. Theileria ovis, B. ovis and B. ovis+T. ovis were found in 15 (83.3%), in 1 (5.6%) and in 2 (11.1%) of 18 Catch-all positive goats respectively. Totally, it was detected B. ovis in 4 (3.4%), T. ovis in 106 (90.6%) and B. ovis+T. ovis in 7 (6%) of 117 Catch-all positive small ruminants. As a result, molecular prevalence was determined in sheep 4% (8/200), in goats 3% (3/100) and in the total 3.6% (11/300) for B. ovis; in sheep 48% (96/200), in goats 17% (17/100) and in the total 37.6 % (113/300) for T. ovis, but B. motasi and B. crassa were not found in this study

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