Özet:

Aim: The aim of the study was to investigate the presence of free-living amoebae (FLA) such as Acanthamoeba and Vermamoeba in air conditioning systems of Cumhuriyet University Research and Application Hospital (CURAH). Methods: For this purpose, twenty-four dust samples were collected using sterile cotton swaps from grilles and filters of five different unit air-conditioners of CURAH located in Sivas, Turkey. Samples were transferred in a sterile tubes to the laboratory and spread onto non-nutrient agar (NNA) plates with a lawn of inactive Escherichia coli. Culture plates were incubated at 30oC.  FLA genera was identified according to morphotyping keys. Results: FLA were found in 14 (58.3%) of examined 24 air-conditioner. Ten (41.6%)were identified as Vermamoeba spp, 4 (16.7%) as Acanthamoeba spp.,  after microscobic examination.   Conclusion: These findings should alert us on the risk of Acanthamoeba and Vermamoeba infections. In addition, these amoebas could be host for bacteria, fungi and viruses and indirectly become agents of transmission for these pathogens. For decrease the risk both Acanthamoeba and their endosymbionts, regularly cleaning of air-conditioner filters and grilles using effective disinfectants are essential.

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