It is known that the intake of trans fats, commonly used in the food industry around the world, has a negative effect on healthy and balanced nutrition. The aim of this study was to determine the biochemical changes in an organism following the consumption of cotton oil (CO) or trans-fat obtained from cotton oil (TF). A total of 18 Wistar Albino male rats were used. The research was performed in SUDAM unit. Divided into 3 equal groups. The control (C) group was fed with a standard rat diet, the CO group was given the standard diet added with 5% CO, and the TF group was given the standard diet added with 5% TF (including 30% trans fat). Ad libitum feeding and water were allowed for 8 weeks of the experimental period. Blood samples were collected on Day 0 by the supraorbital method and on Day 60 by the intracardiac method under anesthesia. Glucose, HbA1c, triglyceride, cholesterol, HDL, LDL, AST, ALT, ALP, GGT, and SOD analyzes were performed. SPSS (19.0) program was used for the statistical analyses. Trans fat feeding increased LDL and triglyceride concentrations, decreased HDL concentration, and caused some changes in the levels of the liver enzymes investigated.
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