Özet:

In this study, we investigated the alteration of isolated aorta responses and the relevant relaxingeffects of allopurinol in primary and secondary phases of rat’s adjuvant arthritis model. The arthritiswas evaluated by scoring prior to and 6 (primary phase) and 15 days (secondary phase) afterinduction of arthritis by intradermal Freund’s complete adjuvant. Moreover, the relaxation andcontraction responses of thoracic aorta rings were also recorded in both phases of arthritis. Thecontractile response to phenylephrine in secondary phase was higher than the control and primaryphase. The reduction of the relaxing effect of acetylcholine in all arthritis groups indicatesprofound endothelial disturbance. No difference was found among the groups with regard to theeffects of sodium nitroprusside in the aorta with intact endothelium. Allopurinol alone relaxed theaortic preparation. However, the effect of allopurinol in secondary phase aorts, both with and withoutendothelium, was decreased compared to that obtained in control and primary phase.Therelaxing effect of acetylcholine in presence of allopurinol in aortic preparation with intact endotheliumwas decreased in control and primary phase, but the effect was not changed in secondaryphase. L-NAME altered the effect of allopurinol on acetylcholine. In conclusion, the effect of allopurinolin inflammatory arthritis is partly via an effect on xanthine oxidase and partly on nitric oxide.

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