Özet:

OBJECTIVE: Essential hypertension is defined as high blood pressure with unknown cause or does not due to secondary disease. There are described a lot of gene polymorphisms, metabolic pathways and systems associated with essential hypertension. However these genes, metabolic pathways and systems give partial information for understanding of essential hypertension’s pathogenesis. CHGA gene, one of susceptibility genes, has been studied recently. CHGA gene, which is mainly expressed in the adrenal medulla and postganglionic sympathetic axon, regulates storage and release of catecholamines by intracellular and extracellular mechanisms. In this study, it is aimed to investigate polymorphisms in CHGA gene’s promoter T-1014C, T-988G, G-462A and T-415C regions with sequence analysis in the patients of essential hypertension and control cases.METHODS: In this study, peripheral blood samples were collected by EDTA tubes from 50 patients diagnosed with essential hypertension and 32 healthy controls. DNA was obtained from the samples. DNA sequence with T-1014C, T-988G, S-462 and C-415T polymorphisms at the promoter region of CHGA gene was amplified by polymerase chain reaction (PCR) using specific primers. Polymorphisms were investigated by DNA sequence analysis method using automatic capillary gel electrophoresis instrument. Data were evaluated by descriptive statistical analysis and chi-square tests at SSPS 11.0 versionRESULTS: According to the results in the CHGA gene; frequency of C allele, CC and TC genotypes for promoter T-1014C polymorphic region, G allele, GG and TG genotype for promoter T-988G polymorphic region is significantly higher (p0.05). CONCLUSION: The polymorphisms of T-1014C and T-988G at promoter region of CHGA gene were statistically significant between patient and control groups. The results may not fully reflect CHGA promoter region polymorphisms of Turkish population due to limitations in the number of cases. This study is the first study conducted in Turkish population and it is pioneer for future studies.

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