Abstract:

The objective of this study is to evaluate the antimicrobial activity of punikalagine alone and in combination with cholistine on cholistine-resistant A. baumannii juices. In the study, five colist-resistant A. baumannii juice were used to be tested. Minimum Inhibition Concentrations (MIC) are determined by conducting a fluid microdilusion test to determine the antimicrobial activities of punicalagin and colistine against the clots. The combined activities of these two agencies were evaluated by the Dama Table (Checkerboard) Synergy Test. According to the results of the fluid microdilusion test, the MIC of punikalagine against all juices was 256 μg/ml, while the MIC of cholistine was 32 μg/ml in two juices, and 8 μg/ml in the other three juices. The Checkerboard synergy test and the combination interactions are calculated by the Fraksial Inhibitor Concentration Index (FIC). According to this, the FICI values of the combination of colystin and punikalagin were 1.12 for two and 1.03 for the other three. As a result, punikalagin has shown an antimicrobial activity on the A. baumannii acids resistant to cholistine. The combination of colystin with it has been found to have an indifferent effect on all the tested juices. No synergistic effect was observed on any soil. There is no antagonistic effect.

Keywords:

Abstract:

The aim of this study was to evaluate the antimicrobial activity of punicalagin alone and in combination with colistin on colistin-resistant A. baumannii strains. Five colistin-resistant A. baumannii strains were used to be tested in the study. Broth microdilution test was performed to evaluate the antimicrobial activities of punicalagin and colistin against strains and Minimum Inhibition Concentrations (MIC) were determined. Combination activities of these two agents were evaluated with the Checkerboard Synergy Test. According to the results of the broth microdilution test, the MIC value of punicalagin against all strains was 256 µg/ml, the MIC values of colistin were found to be 32 µg/ml on two strains and 8 µg/ml on the other three strains. The Fractional Inhibitory Concentration Index (FICI) was calculated to determine combination interactions with the Checkerboard synergy test. Accordingly, the FICI values of the combination of colistin and punicalagin were found as 1.12 for two strains, 1.03 for the other three strains. In conclusion, punicalagin showed antimicrobial activity on colistin-resistant A. baumannii strains. It was determined that the combination with colistin had an indifferent effect on all tested strains. No synergistic effects were observed on any of the strains. In addition, no antagonistic effects were detected.